Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy

  • Elisavet VasilopoulouEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2067)


Diabetic nephropathy is associated with injury and loss of podocytes, specialized epithelial cells that are critical for glomerular filtration. This chapter describes a method of isolating and culturing podocyte cells from mouse adult kidneys. In this way, podocytes with genetic modifications can be obtained from transgenic animals and they can be used to study the effects of the diabetic environment in vitro.

Key words

Diabetes Diabetic nephropathy Glomerulus Kidney Podocyte 



This work was supported by a Kidney Research UK Post-Doctoral Fellowship (PDF8/2015) to EV.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Medway School of PharmacyUniversity of KentChathamUK
  2. 2.Developmental Biology and CancerUCL GOS Institute of Child HealthLondonUK

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