Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic Rodent Model

  • Carolynn Cairns
  • Bryan ConwayEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2067)


Traditional animal models mimic only the earliest stages of human diabetic nephropathy (DN), which limits their utility to dissect the pathogenesis of progressive disease or test novel therapeutics. In this chapter we describe in detail the experimental procedures required to conduct the Cyp1a1mRen2 rodent model, in which hyperglycemia and renin-dependent hypertension synergize to promote moderate proteinuria, renal fibrosis, and induction of many of the transcriptomic changes observed in the kidney of patients with progressive DN.

Key words

Animal model Diabetes Hypertension Nephropathy Renin 



Dr. Conway was supported by a MRC Clinician Scientist Award, a Scottish Senior Clinical Fellowship, a British Heart Foundation Transition Fellowship Award, and grants from Kidney Research UK and the Edinburgh and Lothians Health Foundation Renal Fund.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Centre for Cardiovascular ScienceUniversity of EdinburghEdinburghUK

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