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Assessment of Urinary MicroRNAs by Quantitative Polymerase Chain Reaction in Diabetic Nephropathy Patients

  • Lucy Jade Newbury
  • Alexa Wonnacott
  • Kate Simpson
  • Timothy Bowen
  • Donald FraserEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2067)

Abstract

Urinary microRNAs show promise as noninvasive biomarkers in renal disease. Here, we describe a detailed protocol for the column-based extraction and quantification of miRNAs by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) from urine samples.

Key words

MicroRNAs Urine Biomarker Diabetic nephropathy Chronic kidney disease RT-qPCR 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Lucy Jade Newbury
    • 1
  • Alexa Wonnacott
    • 1
  • Kate Simpson
    • 1
  • Timothy Bowen
    • 1
  • Donald Fraser
    • 1
    Email author
  1. 1.Wales Kidney Research UnitHeath Park CampusCardiffUK

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