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Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches

  • Laura J. Smyth
  • Katie Kerr
  • Seamus Duffy
  • Jill Kilner
  • Amy Jayne McKnightEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2067)

Abstract

Multiple genetic strategies are available to help improve understanding of diabetic nephropathy. This methods chapter provides an overview of phenotype considerations specific to diabetic nephropathy and biobank essentials, and provides detailed methodology for a common benchtop wet-lab approach (Ion Torrent semiconductor sequencing) including in silico genetic variant identification from next-generation sequencing data to identify genetic risk factors for diabetic nephropathy.

Key words

Association Diabetic nephropathy Genome-wide Illumina Next-generation sequencing Kidney S5XL SNP Torrent Trend Variant 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Laura J. Smyth
    • 1
  • Katie Kerr
    • 1
  • Seamus Duffy
    • 1
  • Jill Kilner
    • 1
  • Amy Jayne McKnight
    • 1
    Email author
  1. 1.Centre for Public Health, Queen’s University BelfastNorthern IrelandUK

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