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Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging

  • Dhaka Ram Bhandari
  • Giulia Coliva
  • Maria Fedorova
  • Bernhard SpenglerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2064)

Abstract

Complementary to other imaging techniques, MALDI MS imaging provides label-free analysis and chemical specificity, covering a wide range of molecules that can be visualized simultaneously. Here, we highlight a protocol to visualize lipids from single cardiac cells using high-resolution atmospheric-pressure matrix-assisted laser desorption/ionization coupled to an orbital trapping mass analyzer.

Key words

High-resolution atmospheric-pressure mass spectrometry imaging Cardiac cells Single cell imaging 

Notes

Acknowledgments

Financial support by the Deutsche Forschungsgemeinschaft (DFG) under grant Sp314/13-1. M.F. and G.C. are financially supported by funding from the European Commission’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement number 675132 (MSCA-ITN-ETN MASSTRPLAN). Financial support from the German Federal Ministry of Education and Research (BMBF) within the framework of the e:Med research and funding concept for SysMedOS project (to MF) is gratefully acknowledged.

Competing financial interests: B.S. is a consultant to TransMIT GmbH Giessen. D.B. is a part-time employee of TransMIT GmbH.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Dhaka Ram Bhandari
    • 1
  • Giulia Coliva
    • 2
    • 3
  • Maria Fedorova
    • 2
    • 3
  • Bernhard Spengler
    • 1
    Email author
  1. 1.Institute of Inorganic and Analytical ChemistryJustus Liebig University GiessenGiessenGermany
  2. 2.Faculty of Chemistry and Mineralogy, Institute of Bioanalytical ChemistryUniversity of LeipzigLeipzigGermany
  3. 3.Center for Biotechnology and BiomedicineUniversität LeipzigLeipzigGermany

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