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Plant Meiosis pp 359-366 | Cite as

Analyzing Somatic DNA Repair in Arabidopsis Meiotic Mutants

  • Annika Dorn
  • Holger PuchtaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2061)

Abstract

Meiotic and somatic recombination share a common set of factors. Thus, the analysis of somatic DNA repair in meiotic mutant lines should be of special interest. Growth defects of mutant plants induced by specific genotoxins can thereby hint to DNA repair functions of the affected proteins. Here, we describe two kinds of approaches to characterize deficiencies in DNA repair in mutant lines of Arabidopsis thaliana, after genotoxin treatment.

Key words

Arabidopsis thaliana Cisplatin DNA repair Genotoxin Methyl methanesulfonate Mitomycin C Root meristem 

Notes

Acknowledgments

This work was supported by the European Research Council (Advanced Grant “COMREC” and “CRISBREED”).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Botanical InstituteKarlsruhe Institute of TechnologyKarlsruheGermany

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