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Plant Meiosis pp 259-265 | Cite as

How to Study the Proteomes and Phosphoproteomes of Anther and Pollen

  • Chi Zhang
  • Xiaobing Feng
  • Menghui Hu
  • Zaibao Zhang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2061)

Abstract

Proteomics analysis was a powerful technology for characterizing proteins and protein posttranslational modification (PTMs). Recently, many anther and pollen-related proteomic analyses have been reported, which have expanded our understanding of anther and pollen development and regulation. In this chapter, we describe a detailed, optimized protocol for the separation, digestion, tagging, and subsequent mass spectrometry-based identification and quantification of proteins and phosphoproteins from anther and pollen.

Key words

Anther Liquid chromatography–mass spectrometry (LC-MS/MS) Pollen Proteome Phosphoproteome 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Chi Zhang
    • 1
  • Xiaobing Feng
    • 1
  • Menghui Hu
    • 1
  • Zaibao Zhang
    • 1
    • 2
  1. 1.College of Life ScienceXinyang Normal UniversityXinyangChina
  2. 2.Institute for Conservation and Utilization of Agro-Bioresources in Dabie MountainsXinyangChina

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