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Plant Meiosis pp 237-258 | Cite as

Isolating Male Meiocytes from Maize and Wheat for “-Omics” Analyses

  • Stefanie Dukowic-SchulzeEmail author
  • Nelson Garcia
  • Arun S. K. Shunmugam
  • Sateesh Kagale
  • Changbin Chen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2061)

Abstract

Genome-wide gene expression studies have become a routine approach due to the advances in sequencing technologies, their ease of use, and increasing affordability. Simultaneous investigation of small RNA expression adds further valuable information but is not adopted as widely yet. Both RNA-seq and small RNA-seq benefit from the use of specific cell types. Here, we describe a protocol for the isolation of male meiotic cells from maize or wheat plants, along with the application of downstream RNA sequencing, extendable to other -omics approaches.

Key words

Meiocytes Meiosis Maize Wheat Plants Omics Sequencing 

Notes

Acknowledgments

This work was supported by the National Science Foundation (IOS: 1025881, IOS-1546792), a grant-in-aid fund from the University of Minnesota, and Genome Canada funded Canadian Triticum Applied Genomics2 (CTAG2) initiative.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Stefanie Dukowic-Schulze
    • 1
    Email author
  • Nelson Garcia
    • 1
  • Arun S. K. Shunmugam
    • 2
  • Sateesh Kagale
    • 2
  • Changbin Chen
    • 1
  1. 1.Department of Horticultural ScienceUniversity of MinnesotaSaint PaulUSA
  2. 2.National Research Council CanadaSaskatoonCanada

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