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Plant Meiosis pp 169-180 | Cite as

Multicolored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids

  • Virginie Huteau
  • Olivier CoritonEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2061)

Abstract

Genetic diversity can be introduced into polyploid crop species through meiotic recombination by exchanges between homologous or homoeologous chromosomes. Fluorescent in situ hybridization (FISH) enables the characterization of these homologous and homoeologous chromosome pairs during meiosis and identification of structural rearrangements during mitosis in metaphase I. In this chapter, we describe a protocol for the multicolored fluorescent labeling of chromosome spreads. This protocol allows the characterization of each A and C homoeologous subgenomes in a polyploid species using a genome-specific BAC combined with specific chromosome labeling BAC sequences.

Key words

Brassica species Chromosome Cytogenetics Meiosis Mitosis Fluorescence in situ hybridization 

Notes

Acknowledgments

We thank Anne-Marie Chèvre and Julie Ferreira-De-Carvalho for constructive reading of the manuscript.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Institut de Génétique, Environnement et Protection des PlantesINRA, UMR 1349 IGEPP, Molecular cytogenetics Platform, BP35327, F-35653Le Rheu CedexFrance

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