Analysis of Meiosis in Nonmodel Tropical Plants: The Case of Carica papaya Linn
To develop plants that are more tolerant to drought, marginal soil fertility, and diseases and that satisfy demands for high yield, new cultivars of the tropical fruit papaya (Carica papaya L.) are needed. Nonetheless, in many cases, these traits are available in only wild relatives found throughout Latin America. Understanding meiotic progression may facilitate the introgression of desirable traits into commercial cultivars that maintain high fertility. In this protocol, we describe a practical and simple method to effectively isolate male meiocytes in order to document the behavior of papaya meiotic chromosomes.
Key wordsMeiosis Nonmodel organism Papaya Plant chromosomes Tropical agriculture
Our work was supported by the Vicerrectoría de Investigación intramural grants # B5A13 and B0185, and we particularly wish to thank Vicechancellor Fernando García-Santamaría. Our Dell Precision Tower 7820 computer system was kindly donated by the Spanish Agency for International Development Cooperation (Agencia Española de Cooperación Internacional para el Desarrollo). We thank previous students Stefano Albertazzi, Sergio Castro-Pacheco (now a student at the Erasmus Mundus Master in Plant Breeding program), and Romano Porras (now at Universität Hohenheim, Stuttgart, Germany) for their valuable work. We also thank Eric Mora-Newcomer and Walter Barrantes-Santamaría for providing flower samples from their papaya breeding program. Pablo is a young member affiliate of the World Academy of Sciences (TWAS/UNESCO) and a member of the American Society of Plant Biologists.
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