Herpes Simplex Virus Growth, Preparation, and Assay

  • Sereina O. Sutter
  • Peggy Marconi
  • Anita F. MeierEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2060)


The human herpesvirus family members, in particular herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2), are abundant and extremely contagious viruses with a high seroprevalence in the human population emphasizing the importance of studying their biology. Hence, the propagation and purification of virus stocks constitute a key element in laboratory work.

Key words

HSV growth Plaque purification Plaque titration Growth curve Virus stock Purification 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Sereina O. Sutter
    • 1
  • Peggy Marconi
    • 2
  • Anita F. Meier
    • 1
    Email author
  1. 1.Institute of Virology, Vetsuisse FacultyUniversity of ZurichZürichSwitzerland
  2. 2.Department of Chemical and Pharmaceutical Sciences (DipSCF)University of FerraraFerraraItaly

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