The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines

  • Joshua O. Marshak
  • Lichun Dong
  • David M. KoelleEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2060)


DNA vaccines have been licensed in veterinary medicine and have promise for humans. This format is relatively immunogenic in mice and guinea pigs, the two principle HSV-2 animal models, permitting rapid assessment of vectors, antigens, adjuvants, and delivery systems. Limitations include the relatively poor immunogenicity of naked DNA in humans and the profound differences in HSV-2 pathogenesis between host species. Herein, we detail lessons learned investigating candidate DNA vaccines in the progesterone-primed female mouse vaginal model of HSV-2 infection as a guide to investigators in the field.

Key words

Herpes simplex virus Animal model DNA vaccine Antibody Polymerase chain reaction Latency Dorsal root ganglia 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Joshua O. Marshak
    • 1
  • Lichun Dong
    • 1
  • David M. Koelle
    • 1
    • 2
    • 3
    • 4
    • 5
    Email author
  1. 1.Department of MedicineUniversity of WashingtonSeattleUSA
  2. 2.Department of Laboratory MedicineUniversity of WashingtonSeattleUSA
  3. 3.Department of Global HealthUniversity of WashingtonSeattleUSA
  4. 4.Vaccine and Infectious Diseases DivisionFred Hutchinson Cancer Research CenterSeattleUSA
  5. 5.Benaroya Research InstituteSeattleUSA

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