The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1

  • Kevin Danastas
  • Anthony L. Cunningham
  • Monica Miranda-SaksenaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2060)


Understanding how herpes simplex virus-1 (HSV-1) interacts with different parts of the neuron is fundamental in understanding the mechanisms behind HSV-1 transport during primary and recurrent infections. In this chapter, we describe a unique neuronal culture system that is capable of compartmentalizing neuronal cell bodies from their axons to study the transport of HSV-1 along axons. The ability to separate neuronal cell bodies and axons provides a unique model to investigate the mechanisms used by HSV-1 for viral transport, assembly, and exit from different parts of the neuron.

Key words

Microfluidics Neurons Herpes simplex virus Axons Transport 



This work was supported by the Australian National Health and Medical Research Grants (APP1069193 and APP1130512).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Kevin Danastas
    • 1
    • 2
  • Anthony L. Cunningham
    • 1
    • 3
  • Monica Miranda-Saksena
    • 1
    • 2
    Email author
  1. 1.Centre for Virus ResearchThe Westmead Institute for Medical ResearchWestmeadAustralia
  2. 2.The University of SydneyWestmeadAustralia
  3. 3.Sydney Medical SchoolThe University of SydneyWestmeadAustralia

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