Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as Indicators of Plant Stress Response

  • Alexander T. Eprintsev
  • Natalia V. Selivanova
  • Abir U. IgamberdievEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2057)


Glutamate plays a central role in amino acid metabolism, in particular, in aminotransferase reactions leading to formation of many other proteinogenic and nonproteinogenic amino acids. In stress conditions, glutamate can be either metabolized to γ-aminobutyric acid (GABA) by glutamate decarboxylase which initiates a GABA shunt bypassing several reactions of the tricarboxylic acid cycle or converted to 2-oxoglutarate by glutamate dehydrogenase. Both reactions direct protein carbon to respiration but also link glutamate metabolism to other cellular pathways, resulting in the regulation of redox level and pH balance. Assays for determination of activities of glutamate dehydrogenase and of the GABA shunt enzymes as the markers of stress response is described in this chapter. These assays are important in the studies of the strategy of biochemical adaptation of plants to changing environmental conditions including elevated CO2, temperature increase, flooding, and other stresses.

Key words

Amino acid metabolism γ-aminobutyric acid (GABA) Glutamate Respiration Stress 



Funding: This work was supported by the grant 19-14-00150 of the Russian Science Foundation.


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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alexander T. Eprintsev
    • 1
  • Natalia V. Selivanova
    • 1
  • Abir U. Igamberdiev
    • 2
    Email author
  1. 1.Department of Biochemistry and Cell PhysiologyVoronezh State UniversityVoronezhRussia
  2. 2.Department of BiologyMemorial University of NewfoundlandSt. John’sCanada

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