Methods for Estimation of Nitrogen Components in Plants and Microorganisms

  • Pratiksha Singh
  • Rajesh Kumar Singh
  • Qi-Qi Song
  • Hai-Bi Li
  • Li-Tao Yang
  • Yang-Rui Li
Part of the Methods in Molecular Biology book series (MIMB, volume 2057)


Nitrogen (N2) is the most necessary element in the atmosphere, it is an energetic micronutrient for plant growth and development after water, besides its key role in chlorophyll production, which is crucial for photosynthesis process. Biological nitrogen fixation is measured to be the most potent method to deliver a fixed way of nitrogen to the plants. Plant depends on free-living and symbiotic microbes present in the soil for nitrogen because it cannot be absorbed by the plant itself directly from the atmosphere. Many techniques were reported in the laboratory for nitrogen estimation till now, but Kjeldahl digestion and acetylene reduction assay (ARA) techniques became the most popular. In this chapter, we focus on the most common and popular methods used to determine plant N2; awareness obtained through the wide application of these methods should offer the source for the N2 fixation rate in agriculture system.

Key words

Acetylene reduction assay Combustion method Kjeldahl Microbial bioassay 15N2 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Pratiksha Singh
    • 1
    • 2
    • 3
  • Rajesh Kumar Singh
    • 1
    • 2
    • 3
  • Qi-Qi Song
    • 1
    • 2
  • Hai-Bi Li
    • 1
    • 2
  • Li-Tao Yang
    • 1
    • 2
  • Yang-Rui Li
    • 1
    • 2
  1. 1.Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Guangxi key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research InstituteGuangxi Academy of Agricultural SciencesNanningChina
  2. 2.College of AgricultureGuangxi UniversityNanningChina
  3. 3.Guangxi key Laboratory of Crop Genetic Improvement and BiotechnologyNanningChina

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