Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae
Instability of trinucleotide and inverted repeats is a causative factor in the development of a number of neurological diseases, hereditary syndromes, and cancer. To understand the mechanisms that lead to repeat-induced genome destabilization it is important to identify factors that affect repeat metabolism. Here we present an approach that utilizes systematic and unbiased genome-wide screen in yeast Saccharomyces cerevisiae aimed to find genes that govern GAA/TTC and inverted repeat instability. These screens allowed for the identification of more than 30 mutants with increased fragility of both repeat motifs.
KeywordsTrinucleotide repeats Inverted repeats Genome instability Double-strand breaks Gross chromosomal rearrangements Genome-wide screen
We thank Dr. N. Degtyareva for critical reading of the manuscript and Dr. Y. Zhang for carrying out the screens. This work was supported by NIH grant R01GM129119 to KSL.
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