Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model
Analysis of individual gametes has a number of applications in the study of the mechanism of repeat expansion in mouse models of the fragile X-related disorders, as well as in mouse models of other repeat expansion diseases. This chapter describes the techniques required to isolate oocytes and male gametes of different stages of maturity, along with the techniques required to accurately determine the repeat number in these gametes.
KeywordsOocyte Sperm Germ cell Fluorescence-activated cell sorting Single cell analysis Small pool PCR
The authors would like to thank Dr. John Philip McCoy, Jr. (NHLBI) for his careful reading of the manuscript. This work was made possible with funding to KU from the Intramural Program of NIDDK, NIH (DK057808).
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