Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection

  • Anson V. KoehlerEmail author
  • Jan ŠlapetaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2052)


Polymerase chain reaction (PCR) enables amplification of specific DNA fragments for the detection and tracking of Cryptosporidium spp. Newly obtained DNA are compared to an ever-growing database of Cryptosporidium sequences with uncertain or outdated metadata in primary public repositories (i.e., EMBL/DDBJ/GenBank). Here, we describe standard operating procedures to obtain DNA sequences from Cryptosporidium spp. marker genes. Small-subunit ribosomal RNA gene, large-subunit ribosomal RNA gene, and glycoprotein 60 are amplified using conventional PCR. Amplified and sequenced genes are compared to a reference library of up-to-date curated gene sequences to identify Cryptosporidium species and variants.


PCR DNA Genotyping LSU SSU gp60 Cryptosporidium 


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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural SciencesThe University of MelbourneParkvilleAustralia
  2. 2.Sydney School of Veterinary Science, Faculty of ScienceThe University of SydneySydneyAustralia

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