Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration
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The procedure described here provides instructions for detection of Cryptosporidium recovered from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium DNA.
KeywordsCryptosporidium Dead-end ultrafiltration IMS Microscopy Real-time PCR Water
Disclosures: Use of trade names is for identification only and does not imply endorsement by the Public Health Service or by the U.S. Department of Health and Human Services. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention.
- 3.Raphael BH, Lautenschlager M, Kahler A, Pai S, Parks BA, Kalb SR, Maslanka SE, Shah S, Magnuson M, Hill VR (2012) Ultrafiltration improves ELISA and Endopep MS analysis of botulinum neurotoxin type A in drinking water. J Microbiol Methods 90(3):267–272. https://doi.org/10.1016/j.mimet.2012.05.020CrossRefPubMedGoogle Scholar
- 4.USEPA (2012) Method 1623.1: Cryptosporidium and Giardia in water by filtration/IMS/FA. Office of Water, Washington, D.CGoogle Scholar