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Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration

  • Amy M. KahlerEmail author
  • Vincent R. Hill
Protocol
  • 596 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2052)

Abstract

The procedure described here provides instructions for detection of Cryptosporidium recovered from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium DNA.

Keywords

Cryptosporidium Dead-end ultrafiltration IMS Microscopy Real-time PCR Water 

Notes

Acknowledgments

Disclosures: Use of trade names is for identification only and does not imply endorsement by the Public Health Service or by the U.S. Department of Health and Human Services. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention.

References

  1. 1.
    Mull B, Hill VR (2012) Recovery of diverse microbes in high turbidity surface water samples using dead-end ultrafiltration. J Microbiol Methods 91(3):429–433.  https://doi.org/10.1016/j.mimet.2012.10.001CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Smith CM, Hill VR (2009) Dead-end hollow-fiber ultrafiltration for recovery of diverse microbes from water. Appl Env Microbiol 75(16):5284–5289.  https://doi.org/10.1128/AEM.00456-09CrossRefGoogle Scholar
  3. 3.
    Raphael BH, Lautenschlager M, Kahler A, Pai S, Parks BA, Kalb SR, Maslanka SE, Shah S, Magnuson M, Hill VR (2012) Ultrafiltration improves ELISA and Endopep MS analysis of botulinum neurotoxin type A in drinking water. J Microbiol Methods 90(3):267–272.  https://doi.org/10.1016/j.mimet.2012.05.020CrossRefPubMedGoogle Scholar
  4. 4.
    USEPA (2012) Method 1623.1: Cryptosporidium and Giardia in water by filtration/IMS/FA. Office of Water, Washington, D.CGoogle Scholar
  5. 5.
    Hill V, Narayanan J, Gallen R, Ferdinand K, Cromeans T, Vinje J (2015) Development of a nucleic acid extraction procedure for simultaneous recovery of DNA and RNA from diverse microbes in water. Pathogens 4:335–354CrossRefGoogle Scholar
  6. 6.
    Jothikumar N, da Silva AJ, Moura I, Qvarnstrom Y, Hill VR (2008) Detection and differentiation of Cryptosporidium hominis and Cryptosporidium parvum by dual TaqMan assays. J Med Microbiol 57(9):1099–1105.  https://doi.org/10.1099/jmm.0.2008/001461-0CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Division of Foodborne, Waterborne, and Environmental DiseasesNational Center for Emerging and Zoonotic Infectious Diseases, Centers for Diseases Control and PreventionAtlantaUSA

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