Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration

  • Amy M. KahlerEmail author
  • Vincent R. Hill
Part of the Methods in Molecular Biology book series (MIMB, volume 2052)


The procedure described here provides instructions for detection of Cryptosporidium recovered from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium DNA.


Cryptosporidium Dead-end ultrafiltration IMS Microscopy Real-time PCR Water 



Disclosures: Use of trade names is for identification only and does not imply endorsement by the Public Health Service or by the U.S. Department of Health and Human Services. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Division of Foodborne, Waterborne, and Environmental DiseasesNational Center for Emerging and Zoonotic Infectious Diseases, Centers for Diseases Control and PreventionAtlantaUSA

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