High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR
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An effective method to quantify the parasite loads is the key to the evaluation of anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening (HTS) of drugs against Cryptosporidium parvum in vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth of C. parvum in vitro.
KeywordsCryptosporidium parvum In vitro cell culture Real-time quantitative RT-PCR (qRT-PCR) Drug screening
This research was supported in part by the National Institute of Allergy and Infectious Disease of the National Institutes of Health under award number R21AI099850.
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