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Low-Voltage Flow-Through Electroporation Membrane and Method

  • Juliette Experton
  • Aaron G. Wilson
  • Charles R. Martin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2050)

Abstract

Electroporation uses high electric field gradients to create pores within the membrane of living cells in order to deliver a substance, for example a gene, into the cytoplasm. To achieve such gradients, current electroporation devices deliver voltage pulses in the kV range to the cell medium. We describe here a new device based on gold-microtube membranes that can accomplish electroporation with voltage pulses that are orders of magnitude smaller, 4 V. The percentages of electroporated bacteria were found to be more than an order of magnitude higher than obtained with a commercial electroporator.

Key words

Electroporation Gold-microtube membranes Escherichia coli Electric field gradient 

Notes

Acknowledgments

This work was supported by the University of Florida.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Juliette Experton
    • 1
  • Aaron G. Wilson
    • 1
  • Charles R. Martin
    • 1
  1. 1.Department of ChemistryUniversity of FloridaGainesvilleUSA

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