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Ultrahigh-Density Screens for Genome-Wide Yeast EMAPs in a Single Plate

  • Katherine Licon
  • John Paul Shen
  • Brenton P. Munson
  • Manuel Michaca
  • Cole Fassino
  • Luke Fassino
  • Jason F. Kreisberg
  • Trey IdekerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2049)

Abstract

Systematic measurements of genetic interactions have been used to classify gene functions and to categorize genes into protein complexes, functional pathways and biological processes. This protocol describes how to perform a high-throughput genetic interaction screen in S. cerevisiae using a variant of epistatic miniarray profiles (E-MAP) in which the fitnesses of 6144 colonies are measured simultaneously. We also describe the computational methods to analyze the resulting data.

Key words

EMAP Genetic interactions Synthetic lethality Ultrahigh-density yeast arrays S-scores 

Notes

Acknowledgments

This work was supported by NIH grants L30 CA171000 to J.P.S. and R01 GM084279, R01 ES014811, and U54 CA209891 to T.I.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Katherine Licon
    • 1
  • John Paul Shen
    • 1
  • Brenton P. Munson
    • 1
    • 2
  • Manuel Michaca
    • 1
  • Cole Fassino
    • 1
  • Luke Fassino
    • 1
  • Jason F. Kreisberg
    • 1
  • Trey Ideker
    • 1
    • 2
    Email author
  1. 1.Department of MedicineUC San DiegoLa JollaUSA
  2. 2.Department of BioengineeringUC San DiegoLa JollaUSA

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