Ultrahigh-Density Screens for Genome-Wide Yeast EMAPs in a Single Plate
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Systematic measurements of genetic interactions have been used to classify gene functions and to categorize genes into protein complexes, functional pathways and biological processes. This protocol describes how to perform a high-throughput genetic interaction screen in S. cerevisiae using a variant of epistatic miniarray profiles (E-MAP) in which the fitnesses of 6144 colonies are measured simultaneously. We also describe the computational methods to analyze the resulting data.
Key wordsEMAP Genetic interactions Synthetic lethality Ultrahigh-density yeast arrays S-scores
This work was supported by NIH grants L30 CA171000 to J.P.S. and R01 GM084279, R01 ES014811, and U54 CA209891 to T.I.
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