Protein–Protein Interaction Profiling in Candida albicans Revealed by Biochemical Purification–Mass Spectrometry (BP/MS)
BP/MS is a new experimental proteomic platform for systematic study of native protein complexes and global protein–protein interaction networks based on deep biochemical purification of soluble protein extracts and mass spectrometry identification of coeluting proteins. Herein, we describe the application of this methodology to draft a high-confidence protein interaction network for Candida albicans.
Key wordsProtein–protein interaction Candida albicans High-performance liquid chromatography (HPLC) Ion exchange chromatography (IEX) Nano-liquid chromatography–tandem mass spectrometry (nLC-MS/MS)
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