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Protein–Protein Interaction Profiling in Candida albicans Revealed by Biochemical Purification–Mass Spectrometry (BP/MS)

  • Reza Pourhaghighi
  • Teresa R. O’Meara
  • Leah E. Cowen
  • Andrew EmiliEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2049)

Abstract

BP/MS is a new experimental proteomic platform for systematic study of native protein complexes and global protein–protein interaction networks based on deep biochemical purification of soluble protein extracts and mass spectrometry identification of coeluting proteins. Herein, we describe the application of this methodology to draft a high-confidence protein interaction network for Candida albicans.

Key words

Protein–protein interaction Candida albicans High-performance liquid chromatography (HPLC) Ion exchange chromatography (IEX) Nano-liquid chromatography–tandem mass spectrometry (nLC-MS/MS) 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Reza Pourhaghighi
    • 1
  • Teresa R. O’Meara
    • 2
  • Leah E. Cowen
    • 2
  • Andrew Emili
    • 1
    • 3
    Email author
  1. 1.Donnelly Centre for Cellular and Biomolecular ResearchUniversity of TorontoTorontoCanada
  2. 2.Department of Molecular GeneticsUniversity of TorontoTorontoCanada
  3. 3.Center for Network Systems BiologyBoston UniversityBostonUSA

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