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Generation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells

  • Hideyuki OguroEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2048)

Abstract

Human pluripotent stem cells (PSCs) have the potential to provide a virtually unlimited supply of cells for transplantation therapy. When combined with recent advances in genome editing technologies, human PSCs could offer various approaches that enable gene therapy, drug discovery, disease modeling, and in vitro modeling of human development. De novo generation of hematopoietic stem cells (HSCs) from human PSCs is an important focus in the field, since it enables autologous HSC transplantation to treat many blood disorders and malignancies. Although culture conditions have been established to generate a broad spectrum of hematopoietic progenitors from human PSCs, it remains a significant challenge to generate bona fide HSCs that possess sustained self-renewal and multilineage differentiation capacities upon transplantation. In this review, recent promising advances in the efforts to generate HSCs and hematopoietic progenitors from human PSCs in vitro and in vivo or from somatic cells are discussed.

Key words

Hematopoietic stem cells Hematopoietic progenitors Human pluripotent stem cells Hemogenic endothelium Hematopoietic differentiation Hematopoietic development 

Notes

Acknowledgments

This work was supported by The Jackson Laboratory. The author thanks William Skarnes for thoughtful reading and editing of the manuscript and apologizes to authors whose papers could not be cited in this review owing to space limitations.

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Authors and Affiliations

  1. 1.Cellular EngineeringThe Jackson Laboratory for Genomic MedicineFarmingtonUSA

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