In Vitro Differentiation of T Cells: From Nonhuman Primate-Induced Pluripotent Stem Cells
In this chapter, we describe a protocol for hematopoietic differentiation of nonhuman primate (NHP)-induced pluripotent stem cells (iPSCs) derived from T cells and generation of T cells. Derivation of T cells from PSCs involves three steps: induction of PSCs to hematopoietic progenitor cells (HPCs), differentiation of HPCs into progenitor T cells, and maturation of progenitor T cells into mature T cells, in particular CD8 single-positive (SP) T cells.
Key wordsT cells Nonhuman primate-induced pluripotent stem cells In vitro differentiation Hematopoietic progenitor cells Preclinical model
We thank Mr. Yasuyuki Miyake, Shuichi Kitayama, and Dr. Kahoru Taya for help in the preparation. This work is supported in part by KAKENHI to S.I. and S.K.
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