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In Vitro Differentiation of T Cells: From Nonhuman Primate-Induced Pluripotent Stem Cells

  • Shoichi Iriguchi
  • Shin KanekoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2048)

Abstract

In this chapter, we describe a protocol for hematopoietic differentiation of nonhuman primate (NHP)-induced pluripotent stem cells (iPSCs) derived from T cells and generation of T cells. Derivation of T cells from PSCs involves three steps: induction of PSCs to hematopoietic progenitor cells (HPCs), differentiation of HPCs into progenitor T cells, and maturation of progenitor T cells into mature T cells, in particular CD8 single-positive (SP) T cells.

Key words

T cells Nonhuman primate-induced pluripotent stem cells In vitro differentiation Hematopoietic progenitor cells Preclinical model 

Notes

Acknowledgments

We thank Mr. Yasuyuki Miyake, Shuichi Kitayama, and Dr. Kahoru Taya for help in the preparation. This work is supported in part by KAKENHI to S.I. and S.K.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Research and ApplicationKyoto UniversityKyotoJapan
  2. 2.Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and ApplicationKyoto UniversityKyotoJapan

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