In Vitro Differentiation of T Cell: From CAR-Modified T-iPSC

  • Tatsuki Ueda
  • Shin KanekoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2048)


T cells engineered to express chimeric antigen receptor (CAR) against the B cell antigen CD19 are achieving remarkable clinical effects on hematological malignancies. Allogeneic transplantation approach is promising for broaden application of CART therapy. iPSCs are one of the ideal cell sources for this approach. CAR-engineered iPSCs are demonstrated to give rise to CAR-engineered T cell and exert their effector function. In this section, we describe the method to generate CAR-engineered iPSCs and differentiate them into T cells.

Key words

Chimeric antigen receptor Induced pluripotent stem cells Immunotherapy T cell differentiation Lentiviral transduction 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Research and ApplicationKyoto UniversityKyotoJapan
  2. 2.Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and ApplicationKyoto UniversityKyotoJapan

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