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Quantification of Methanogenic Pathways Using Stable Carbon Isotopic Signatures

  • Quan YuanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2046)

Abstract

In many anaerobic environments methane (CH4) is produced by methanogens, with either H2/CO2 or acetate (i.e., the methyl group) as precursors, through what are referred to as hydrogenotrophic and acetoclastic methanogenic pathways respectively. Their relative contribution to total CH4 production can be quantified by determining the stable carbon isotopic fractionation factors for both pathways as well as the isotopic signatures of CO2, CH4, and the methyl group in acetate of the sample. The procedures for measuring carbon isotopic fractionation factors of both methanogenic pathways and isotopic composition of these compounds by isotope ratio mass spectrometry are described in this chapter. The results are very helpful in evaluating the activity of the methanogens involved in each methanogenic pathway as well as those of other biological pathways with different fractionation factors.

Key words

Stable carbon isotope Isotopic fractionation factor Methane Methanogenic pathway GC-C-IRMS 

Notes

Acknowledgments

This work was funded by the National Natural Science Foundation of China (41573083) and State Key Laboratory of Environmental Geochemistry (SKLEG2015403), China.

References

  1. 1.
    Blagodatskaya E, Kuzyakov Y (2013) Active microorganisms in soil: critical review of estimation criteria and approaches. Soil Biol Biochem 67:192–211CrossRefGoogle Scholar
  2. 2.
    Uhlik O, Leewis MC, Strejcek M, Musilova L, Mackova M, Leigh MB, Macek T (2013) Stable isotope probing in the metagenomics era: a bridge towards improved bioremediation. Biotechnol Adv 31:154–165CrossRefGoogle Scholar
  3. 3.
    Ram RJ, VerBerkmoes NC, Thelen MP, Tyson GW, Baker BJ, Blake RC, Shah M, Hettich RL, Banfield JF (2005) Community proteomics of a natural microbial biofilm. Science 308:1915–1920CrossRefGoogle Scholar
  4. 4.
    Simon C, Daniel R (2011) Metagenomic analyses: past and future trends. Appl Environ Microb 77:1153–1161CrossRefGoogle Scholar
  5. 5.
    Conrad R (2005) Quantification of methanogenic pathways using stable carbon isotopic signatures: a review and a proposal. Org Geochem 36:739–752CrossRefGoogle Scholar
  6. 6.
    Conrad R, Claus P, Casper P (2009) Characterization of stable isotope fractionation during methane production in the sediment of a eutrophic lake, Lake Dagow, Germany. Limnol Oceanogr 54:457–471CrossRefGoogle Scholar
  7. 7.
    Blaser M, Conrad R (2016) Stable carbon isotope fractionation as tracer of carbon cycling in anoxic soil ecosystems. Curr Opin Biotech 41:122–129CrossRefGoogle Scholar
  8. 8.
    Hayes JM (1993) Factors controlling C-13 contents of sedimentary organic-compounds—principles and evidence. Mar Geol 113:111–125CrossRefGoogle Scholar
  9. 9.
    Penning H, Conrad R (2007) Quantification of carbon flow from stable isotope fractionation in rice field soils with different organic matter content. Org Geochem 38:2058–2069CrossRefGoogle Scholar
  10. 10.
    Yuan Q, Pump J, Conrad R (2012) Partitioning of CH4 and CO2 production originating from rice straw, soil and root organic carbon in rice microcosms. PLoS One 7:e49073CrossRefGoogle Scholar
  11. 11.
    Yuan Q, Pump J, Conrad R (2014) Straw application in paddy soil enhances methane production also from other carbon sources. Biogeosciences 11:237–246CrossRefGoogle Scholar
  12. 12.
    Krummen M, Hilkert AW, Juchelka D, Duhr A, Schluter HJ, Pesch R (2004) A new concept for isotope ratio monitoring liquid chromatography/mass spectrometry. Rapid Commun Mass Spectrom 18:2260–2266CrossRefGoogle Scholar
  13. 13.
    Penning H, Tyler SC, Conrad R (2006) Determination of isotope fractionation factors and quantification of carbon flow by stable carbon isotope signatures in a methanogenic rice root model system. Geobiology 4:109–121CrossRefGoogle Scholar
  14. 14.
    Mariotti A, Germon JC, Hubert P, Kaiser P, Letolle R, Tardieux A, Tardieux P (1981) Experimental-determination of nitrogen kinetic isotope fractionation—some principles—illustration for the denitrification and nitrification processes. Plant Soil 62:413–430CrossRefGoogle Scholar
  15. 15.
    Janssen PH, Frenzel P (1997) Inhibition of methanogenesis by methyl fluoride: studies of pure and defined mixed cultures of anaerobic bacteria and archaea. Appl Environ Microbiol 63:4552–4557PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Environmental Geochemistry, Institute of GeochemistryChinese Academy of SciencesGuiyangChina

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