SIP-Metaproteomics: Linking Microbial Taxonomy, Function, and Activity
Stable isotope probing combined with metaproteomics enables the detection and characterization of active key species in microbial populations under near-natural conditions, which greatly helps to understand the metabolic functions of complex microbial communities. This is achieved by providing growth substrates labeled with heavy isotopes such as 13C, which will be assimilated into microbial biomass. After subsequent extraction of proteins and proteolytic cleavage into peptides, the heavy isotope enrichment can be detected by high-resolution mass spectrometric analysis, and linked to the functional and taxonomic characterization of these biomarkers. Here we provide protocols for obtaining isotopically labeled proteins and for downstream SIP-metaproteomics analysis.
Key wordsStable isotope probing Metaproteomics Microbial ecology Metabolic labeling Mass spectrometry
I thank Nico Jehmlich for providing helpful inputs and for reviewing the manuscript.
- 10.Dumont MG, Pommerenke B, Casper P (2013) Using stable isotope probing to obtain a targeted metatranscriptome of aerobic methanotrophs in lake sediment. Env Microbiol Rep 5:757–764Google Scholar