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RNA-Based Stable Isotope Probing (RNA-SIP) in the Gut Environment

  • Severin Weis
  • Sylvia Schnell
  • Markus EgertEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2046)

Abstract

The RNA-SIP technology allows for linking the structure and function of complex microbial communities, that is, the identification of microbial key players involved in distinct degradation and assimilation processes under in situ conditions. Being dependent on RNA, this technique is particularly suited for environments with high numbers of very active, that is, significantly RNA-expressing microorganisms, such as intestinal tract samples. We use RNA-SIP for the identification of bacteria involved in the degradation and assimilation of prebiotic carbohydrates in order to better understand the functionality of these medically and economically important nutrients in human and animal intestinal environments.

Key words

RNA-SIP Intestinal tract Digestion Prebiotics Glucose Resistant starch 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Medical and Life Sciences, Institute of Precision Medicine, Microbiology and Hygiene GroupFurtwangen UniversityVillingen-SchwenningenGermany
  2. 2.Institute of Applied Microbiology, Research Center for BioSystems, Land Use, and Nutrition (IFZ)Justus–Liebig–University GiessenGiessenGermany

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