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Automated Planar Patch-Clamp Recording of P2X Receptors

  • Carol J. MilliganEmail author
  • Lin-Hua Jiang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2041)

Abstract

P2X receptors are a structurally and functionally distinctive family of ligand-gated ion channels that play important roles in mediating extracellular adenosine 5′-triphosphate (ATP) signaling in diverse physiological and pathophysiological processes. For several decades, the “manual” patch-clamp technique was regarded as the gold standard assay for investigating ion channel properties. More recently, breakthroughs in the development of automated patch-clamp technologies are enabling the study of ion channels, with much greater throughput capacities. These automated platforms, of which there are many, generate consistent, reliable, high-fidelity data. This chapter demonstrates the versatility of one of these technologies for ligand-gated ion channels, with a particular emphasis on protocols that address some of the issues of receptor desensitization that are commonly associated with P2X receptor-mediated currents.

Key words

Automated electrophysiology Planar patch-clamp Planar chip Microfluidics Stacked solution application Ligand-gated ion channels P2X receptors Voltage-clamp 

Notes

Acknowledgments

Many thanks to Dr. Sonja Stoelzle and Dr. Alison Obergrussberger (Nanion Technologies GmbH) for P2X2/3R data.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.The Florey Institute of Neuroscience and Mental HealthMelbourne Brain CentreParkvilleAustralia
  2. 2.School of Biomedical Sciences, Faculty of Biological SciencesUniversity of LeedsLeedsUK

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