Automated Planar Patch-Clamp Recording of P2X Receptors
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P2X receptors are a structurally and functionally distinctive family of ligand-gated ion channels that play important roles in mediating extracellular adenosine 5′-triphosphate (ATP) signaling in diverse physiological and pathophysiological processes. For several decades, the “manual” patch-clamp technique was regarded as the gold standard assay for investigating ion channel properties. More recently, breakthroughs in the development of automated patch-clamp technologies are enabling the study of ion channels, with much greater throughput capacities. These automated platforms, of which there are many, generate consistent, reliable, high-fidelity data. This chapter demonstrates the versatility of one of these technologies for ligand-gated ion channels, with a particular emphasis on protocols that address some of the issues of receptor desensitization that are commonly associated with P2X receptor-mediated currents.
Key wordsAutomated electrophysiology Planar patch-clamp Planar chip Microfluidics Stacked solution application Ligand-gated ion channels P2X receptors Voltage-clamp
Many thanks to Dr. Sonja Stoelzle and Dr. Alison Obergrussberger (Nanion Technologies GmbH) for P2X2/3R data.
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