Purinergic Signaling pp 275-283 | Cite as
Recording P2X Receptors Using Whole-Cell Patch Clamp from Native Monocytes and Macrophages
Protocol
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Abstract
Investigating ion channels in their native cell type is important when striving to understand their regulation and function, but this comes with added complexities due to the plethora of channels and receptors present. Details of recording ATP-gated ion channels in macrophages are presented together with information on how to prepare the primary cells for electrophysiological analysis.
Key words
Whole-cell patch clamp P2X receptor Macrophage ATP EPC10 P2X7 P2X4 MonocyteReferences
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