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Array-Based Profiling of Proteins and Autoantibody Repertoires in CSF

  • Elisa PinEmail author
  • Ronald Sjöberg
  • Eni Andersson
  • Cecilia Hellström
  • Jennie Olofsson
  • August Jernbom Falk
  • Sofia Bergström
  • Julia Remnestål
  • David Just
  • Peter Nilsson
  • Anna MånbergEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2044)

Abstract

Protein profiling enabled through affinity proteomics represents a powerful strategy for analysis of complex samples such as human body fluids. Cerebrospinal fluid (CSF) is the proximal fluid of the central nervous system and is commonly analyzed in the context of neurological diseases. Through the presence of brain-derived proteins, this fluid can offer insight into the physiological state of the brain. Here, we describe multiplex and flexible protein and autoantibody profiling approaches using suspension bead arrays. Through minimal sample processing, these methods enable high-throughput analysis of hundreds of samples and proteins in one single assay and thereby provide powerful approaches for discovery of disease-associated proteins and autoantigens.

Key words

Protein arrays Antigen arrays Antibody arrays Cerebrospinal fluid Protein profiling Autoantibody profiling Autoantibodies Affinity proteomics 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Elisa Pin
    • 1
    Email author
  • Ronald Sjöberg
    • 1
  • Eni Andersson
    • 1
  • Cecilia Hellström
    • 1
  • Jennie Olofsson
    • 1
  • August Jernbom Falk
    • 1
  • Sofia Bergström
    • 1
  • Julia Remnestål
    • 1
  • David Just
    • 1
  • Peter Nilsson
    • 1
  • Anna Månberg
    • 1
    Email author
  1. 1.Division of Affinity Proteomics, Department of Protein ScienceSciLifeLab, KTH—Royal Institute of TechnologyStockholmSweden

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