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Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay

  • Kevin E. Eboigbodin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2042)

Abstract

Real-time polymerase chain reaction (qPCR) has become a prominent technique in life science research particularly for the detection and monitoring of biomarkers, pathogens, and environmental contaminants. Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are among the major pathogens responsible for sexually transmitted diseases (STDs). Here, multiplex qPCR was utilized for the amplification and detection Chlamydia trachomatis and Neisseria gonorrhoeae within the same reaction tube.

Key words

Amplification Detection Diagnosis Multiplex qPCR Chlamydia trachomatis Neisseria gonorrhoeae 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kevin E. Eboigbodin
    • 1
  1. 1.Research and DevelopmentOrion Diagnostica OyEspooFinland

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