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Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion Based Amplification (mSIBA)

  • Kevin E. Eboigbodin
  • Mark Hoser
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2042)

Abstract

Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are among the most prevalent causes of sexually transmitted infections (STIs) worldwide. Timely and accurate diagnosis plays an important role in deciding appropriate treatment and preventing the spread of the infection. Strand invasion based amplification (SIBA), is an established isothermal nucleic acid amplification method for the rapid and accurate detection of infectious diseases. SIBA was applied for the simultaneous detection of CT and NG in less than 1 h. The multiplex SIBA (mSIBA) method displayed high analytical sensitivity and specificity for the detection of CT and NG. Since the method is performed at low and constant temperature, it can therefore be run on portable instruments. SIBA enables rapid screening for CT and NG within point-of-care or central laboratory settings.

Key words

Detection Diagnosis Strand invasion based amplification Multiplex Polymerase chain Chlamydia trachomatis Neisseria gonorrhoeae 

References

  1. 1.
    Centers for Disease Control and Prevention (2014) Sexually transmitted disease surveillance 2013. U.S. Department of Health and Human Services, Atlanta, p 2014Google Scholar
  2. 2.
    Baugh LR, Hill AA, Brown EL, Hunter CP (2001) Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29(5):e29.  https://doi.org/10.1093/nar/29.5.e29CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Newman L, Rowley J, Vander Hoorn S, Wijesooriya NS, Unemo M, Low N, Stevens G, Gottlieb S, Kiarie J, Temmerman M (2015) Global estimates of the prevalence and incidence of four curable sexually transmitted infections in 2012 based on systematic review and global reporting. PLoS One 10(12):e0143304.  https://doi.org/10.1371/journal.pone.0143304CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Papp JR, Schachter J, Gaydos CA, Van Der Pol B (2014) Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae — 2014. MMWR Recomm Rep 63(RR-02):1–19PubMedCentralGoogle Scholar
  5. 5.
    Van Dyck E, Ieven M, Pattyn S, Van Damme L, Laga M (2001) Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests. J Clin Microbiol 39(5):1751–1756.  https://doi.org/10.1128/jcm.39.5.1751-1756.2001CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Gaydos CA, Quinn TC, Willis D, Weissfeld A, Hook EW, Martin DH, Ferrero DV, Schachter J (2003) Performance of the APTIMA combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens. J Clin Microbiol 41(1):304–309.  https://doi.org/10.1128/jcm.41.1.304-309.2003CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Hoser MJ, Mansukoski HK, Morrical SW, Eboigbodin KE (2014) Strand invasion based amplification (SIBA®): a novel isothermal DNA amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte. PLoS One 9(11):e112656.  https://doi.org/10.1371/journal.pone.0112656CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Eboigbodin KE, Hoser MJ (2016) Multiplex strand invasion based amplification (mSIBA) assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Sci Rep 6:20487.  https://doi.org/10.1038/srep20487CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Eboigbodin KE, Moilanen K, Elf S, Hoser M (2017) Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA® BMC Infectious Diseases 17(1):134. https://doi.org/10.1186/s12879-017-2227-xCrossRefGoogle Scholar
  10. 10.
    Eboigbodin KE, Brummer M, Ojalehto T, Hoser M (2016) Rapid molecular diagnostic test for Zika virus with low demands on sample preparation and instrumentation. Diagn Microbiol Infect Dis 86(4):369–371.  https://doi.org/10.1016/j.diagmicrobio.2016.08.027CrossRefPubMedGoogle Scholar
  11. 11.
    Eboigbodin K, Filén S, Ojalehto T, Brummer M, Elf S, Pousi K, Hoser M (2016) Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B. Appl Microbiol Biotechnol:1–9.  https://doi.org/10.1007/s00253-016-7491-yCrossRefGoogle Scholar
  12. 12.
    Formosa T, Alberts BM (1986) Purification and characterization of the T4 bacteriophage uvsX protein. J Biol Chem 261(13):6107–6118PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kevin E. Eboigbodin
    • 1
  • Mark Hoser
    • 2
  1. 1.Research and DevelopmentOrion Diagnostica OyEspooFinland
  2. 2.Molecular BiologyGeneForm TechnologiesBroadstairsUK

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