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Microglia pp 325-336 | Cite as

Transcriptomics and Live Imaging to Define Functional Phenotypes of Microglia in Pathological Human Tissue

  • Giampaolo Milior
  • Farah Chali
  • Tiffany Dos Santos
  • Juliette Royer
  • Richard Miles
  • Mélanie Morin-BrureauEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2034)

Abstract

Human microglia, as those of rodents, may possess multiple functional phenotypes. Here we present protocols to determine elements of these microglial phenotypes obtained after therapeutic excision of brain tissue from patients with epilepsies of the temporal lobe and cortical gliomas. This technique permits to identify microglia, to determine their shape and expression of state-specific markers, to measure resting and induced motilities, to define a human microglial transcriptome, and to determine how it changes after a seizure.

Key words

Human microglia Transcriptomics Microglial markers Motility Epilepsy 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Giampaolo Milior
    • 2
  • Farah Chali
    • 1
  • Tiffany Dos Santos
    • 1
  • Juliette Royer
    • 1
  • Richard Miles
    • 1
  • Mélanie Morin-Brureau
    • 1
    • 3
    Email author
  1. 1.Sorbonne Université, Inserm UMRS 938, Centre de Recherche Saint-Antoine, CRSAParisFrance
  2. 2.Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, Collège de FranceCNR UMR 7241, INSERM U1050, Labex Memolife, PSL Research UniversityParisFrance
  3. 3.Team Immune System and NeuroinflammationSorbonne Université, Inserm UMRS 938, Centre de Recherche Saint-Antoine, CRSAParisFrance

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