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Labeling Microglia with Genetically Encoded Calcium Indicators

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Microglia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2034))

Abstract

Genetically encoded calcium indicators (GECIs) have become widely used for Ca2+ imaging in cultured cells as well as in living organisms. Transduction of microglia with viral vectors encoding GECIs provides a convenient means to label microglia for in vivo Ca2+ imaging. We describe a method using microglia-specific microRNA-9-regulated viral vector, to label microglial cells with a ratiometric GECI (Twitch-2B). This method enables longitudinal recording of both transient and sustained elevations of Ca2+ in microglia in live animals.

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Acknowledgments

We thank Dr. Wilhelm Pfleging from Karlsruhe Institute of Technology for the preparation of microengineered cover glasses for our project, which was funded by Karlsruhe Nano Micro Facility (KNMF Proposal #2013-009-001195) and Ariane Kaupp for help with graphics. This work was supported by VolkswagenStiftung Grant 90233 and DFG GA654/13-1 to O.G.

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Authors and Affiliations

  1. Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Tübingen, Germany

    Yajie Liang & Olga Garaschuk

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  1. Yajie Liang
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  2. Olga Garaschuk
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Correspondence to Olga Garaschuk .

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Editors and Affiliations

  1. Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Tübingen, Germany

    Olga Garaschuk

  2. Faculty of Biology, Medicine and Health The University of Manchester, , Center for Basic and Translational Neuroscience, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen, Denmark, Achucarro Center for Neuroscience IKERBASQUE Basque Foundation for Science Bilbao, Spain, Manchester, UK

    Alexei Verkhratsky

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Liang, Y., Garaschuk, O. (2019). Labeling Microglia with Genetically Encoded Calcium Indicators. In: Garaschuk, O., Verkhratsky, A. (eds) Microglia. Methods in Molecular Biology, vol 2034. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9658-2_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9658-2_18

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