Measuring Microglial Turnover in the Adult Brain
- 1.3k Downloads
Microglia are the main resident immunocompetent cells of the brain with key roles in brain development, homeostasis, and function. Recent reports have started to shed light on the homeostatic mechanisms regulating the composition and turnover of the microglial population under physiological conditions from development to ageing, but our knowledge of the dynamics of microglia is incomplete. Therefore, it appears relevant to provide a standardized approach to quantify the turnover of microglia, with direct application to create a greater understanding of the dynamics of this cell population, and how it may contribute to the pathogenesis and/or progression of neurological disorders. Here we describe a robust immunohistochemical method to analyze microglial proliferation in mouse brain, aiming at providing a shared and universal approach to analyze microglial dynamics across different laboratories.
Key wordsMicroglia Proliferation Self-renewal Turnover BrdU Immunohistochemistry
The authors were funded by Medical Research Council (MR/K022687/1, MR/P024572/1), and a University of Southampton Vice-Chancellor PhD studentship.
- 1.Ginhoux F, Greter M, Leboeuf M, Nandi S, See P, Gokhan S, Mehler MF, Conway SJ, Ng LG, Stanley ER, Samokhvalov IM, Merad M (2010) Fate mapping analysis reveals that adult microglia derive from primitive macrophages. Science 330(6005):841–845. https://doi.org/10.1126/science.1194637CrossRefPubMedPubMedCentralGoogle Scholar
- 6.Tay TL, Mai D, Dautzenberg J, Fernandez-Klett F, Lin G, Sagar, Datta M, Drougard A, Stempfl T, Ardura-Fabregat A, Staszewski O, Margineanu A, Sporbert A, Steinmetz LM, Pospisilik JA, Jung S, Priller J, Grun D, Ronneberger O, Prinz M (2017) A new fate mapping system reveals context-dependent random or clonal expansion of microglia. Nat Neurosci 20(6):793–803. https://doi.org/10.1038/nn.4547CrossRefGoogle Scholar
- 11.Mandyam CD, Harburg GC, Eisch AJ (2007) Determination of key aspects of precursor cell proliferation, cell cycle length and kinetics in the adult mouse subgranular zone. Neuroscience 146(1):108–122. https://doi.org/10.1016/j.neuroscience.2006.12.064CrossRefPubMedPubMedCentralGoogle Scholar