Microglia pp 207-215 | Cite as

Measuring Microglial Turnover in the Adult Brain

  • Diego Gomez-NicolaEmail author
  • Gemma L. Fryatt
  • Katharine E. Askew
Part of the Methods in Molecular Biology book series (MIMB, volume 2034)


Microglia are the main resident immunocompetent cells of the brain with key roles in brain development, homeostasis, and function. Recent reports have started to shed light on the homeostatic mechanisms regulating the composition and turnover of the microglial population under physiological conditions from development to ageing, but our knowledge of the dynamics of microglia is incomplete. Therefore, it appears relevant to provide a standardized approach to quantify the turnover of microglia, with direct application to create a greater understanding of the dynamics of this cell population, and how it may contribute to the pathogenesis and/or progression of neurological disorders. Here we describe a robust immunohistochemical method to analyze microglial proliferation in mouse brain, aiming at providing a shared and universal approach to analyze microglial dynamics across different laboratories.

Key words

Microglia Proliferation Self-renewal Turnover BrdU Immunohistochemistry 



The authors were funded by Medical Research Council (MR/K022687/1, MR/P024572/1), and a University of Southampton Vice-Chancellor PhD studentship.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Diego Gomez-Nicola
    • 1
    Email author
  • Gemma L. Fryatt
    • 1
  • Katharine E. Askew
    • 1
  1. 1.School of Biological SciencesUniversity of SouthamptonSouthamptonUK

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