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Immunophenotyping Using Dried and Lyophilized Reagents

  • Marc Langweiler
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2032)

Abstract

Antibody reagents that are used for flow cytometry immunophenotyping have traditionally been prepared by combining individual liquid antibody conjugates into mixtures. These cocktails have limited shelf-life, and their preparation is time-consuming and prone to laboratory error. Manufacturers of these reagents, in collaboration with several clinical and research centers, have made advances in constructing dried antibody cocktails which have addressed many of the problems inherent in preparing the liquid cocktails on the lab bench. This chapter discusses methods for the use of dried reagents.

Key words

Flow cytometry Immunophenotyping Harmonization Dried antibody cocktail Lyophilization 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Marc Langweiler
    • 1
  1. 1.AshevilleUSA

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