Phytochromes pp 179-192 | Cite as

Measuring Phytochrome-Dependent Light Input to the Plant Circadian Clock

  • Rachael J. Oakenfull
  • James Ronald
  • Seth J. DavisEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2026)


The circadian clock allows plants to synchronize their internal processes with the external environment. This synchronization occurs through daily cues, one of which is light. Phytochromes are well established as light-sensing proteins and have been identified in forming multiple signaling networks with the central circadian oscillator. However, the precise details of how these networks are formed are yet to be established. Using established promoter-luciferase lines for clock genes crossed into mutant lines, it is possible to use luciferase-based imaging technologies to determine whether specific proteins are involved in phytochrome signaling to the circadian oscillator. The methods presented here use two automated methods of luciferase imaging in Arabidopsis to allow for high-throughput measurement of circadian clock components under a range of different light conditions.


Circadian clock Phytochrome Arabidopsis Luciferase TopCount-luminescence measurement 



This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) award number BB/N018540/1.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Rachael J. Oakenfull
    • 1
  • James Ronald
    • 1
  • Seth J. Davis
    • 1
    Email author
  1. 1.Department of BiologyUniversity of YorkYorkUK

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