Phytochromes pp 169-177 | Cite as

A Simple Method for Quantification of Protochlorophyllide in Etiolated Arabidopsis Seedlings

  • Matthew J. TerryEmail author
  • Sylwia M. Kacprzak
Part of the Methods in Molecular Biology book series (MIMB, volume 2026)


Etiolated seedlings accumulate the chlorophyll biosynthesis intermediate protochlorophyllide (Pchlide) and measuring Pchlide can be important for characterizing photomorphogenic mutants that may be affected in chloroplast development. In this chapter we outline a simple and sensitive method for quantifying Pchlide in extracts of Arabidopsis seedlings using fluorescence spectroscopy. This method can be easily adapted to study chloroplast development in a wide range of plant species.


Tetrapyrroles Chlorophyll synthesis Chloroplast development Etioplasts Fluorescence spectroscopy 



Work on tetrapyrroles in the MJT laboratory has been supported by the UK Biotechnology and Biological Sciences Research Council. SMK was supported by the Gatsby Charitable Foundation. This chapter is dedicated to the memory of Margareta Ryberg.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Biological SciencesUniversity of SouthamptonSouthamptonUK

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