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Purification of Native Flagellin

  • María José González
  • Victoria Iribarnegaray
  • Pablo Zunino
  • Paola Scavone
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2021)

Abstract

Flagella are effective organelles of locomotion and one of several virulence factors in Proteus mirabilis. To study their properties and role in virulence, we describe a protocol to extract and purify the native flagellin of P. mirabilis. Purified flagellin can be visualized by SDS-PAGE or immunoblot and is suitable for downstream applications such as immunization.

Key words

Proteus mirabilis Native flagellin Flagellum SDS-PAGE Western blot 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • María José González
    • 1
  • Victoria Iribarnegaray
    • 1
  • Pablo Zunino
    • 1
  • Paola Scavone
    • 1
  1. 1.Departamento de MicrobiologíaInstituto de Investigaciones Biológicas Clemente EstableMontevideoUruguay

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