Adherence of Proteus mirabilis to Uroepithelial Cells

  • María José González
  • Victoria Iribarnegaray
  • Paola Scavone
  • Pablo Zunino
Part of the Methods in Molecular Biology book series (MIMB, volume 2021)


Bacterial adherence to eukaryotic cells is mediated by different adhesins that can act at different stages in bacteria–host interaction. Abundant evidence has suggested that adherence is critical for infection by bacterial pathogens. Proteus mirabilis is an opportunistic pathogen which frequently infects the human urinary tract, particularly in patients with indwelling urinary catheters. Sequencing of the genome of this pathogen has revealed the existence of a remarkable amount of complete fimbrial operons. In this chapter, we describe in vitro adherence assays of P. mirabilis to uroepithelial cells, which can provide relevant results to assess virulence of uropathogenic strains.

Key words

Proteus mirabilis Uropathogen Urinary tract infection Uroepithelial cells Adherence Virulence 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • María José González
    • 1
  • Victoria Iribarnegaray
    • 1
  • Paola Scavone
    • 1
  • Pablo Zunino
    • 1
  1. 1.Departamento de MicrobiologÚaInstituto de Investigaciones Biológicas Clemente EstableMontevideoUruguay

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