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In Vitro Embryo Production in Sheep

  • S. Mondal
  • A. Mor
  • I. J. Reddy
  • S. Nandi
  • P. S. P. Gupta
  • A. Mishra
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2006)

Abstract

The recent advances in biotechnological research have led to development of many advanced reproductive techniques and biological tools which are set to revolutionize the productive efficiency of livestock species. The development of technology for sequencing of whole genomes and mass screening of gene regulation has widened our approach to genetic profiling and mapping, as well as furthering our understanding of underlying physiological mechanisms. The newer biotechnologies of gene transfer, in vitro fertilization and embryo production, cloning, and stem cell technology have been developed and are being refined with efficiencies suitable for use in animal farming. Efficient in vitro systems for maturing oocytes, fertilizing, and developing embryos have resulted in commercial in vitro production of embryos. Here we describe in vitro maturation, in vitro fertilization, embryo production, embryo culture, and quantitation of gene expression in sheep embryos.

Key words

In vitro maturation In vitro fertilization In vitro embryo production Embryo culture Embryonic gene expression 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • S. Mondal
    • 1
    • 2
  • A. Mor
    • 1
  • I. J. Reddy
    • 1
  • S. Nandi
    • 1
  • P. S. P. Gupta
    • 1
  • A. Mishra
    • 1
  1. 1.ICAR-National Institute of Animal Nutrition and PhysiologyBangaloreIndia
  2. 2.Animal Physiology DivisionICAR-National Institute of Animal Nutrition and PhysiologyBangaloreIndia

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