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Proteomic Response of Host Plants to Citrus tristeza virus

  • Milena Santos Dória
  • Carlos Priminho Pirovani
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2015)

Abstract

Proteomics is an excellent technique for detecting proteins involved in plant responses infected with the Citrus tristeza virus. Here we describe the process to extract proteins to obtain two-dimensional gels with a large number of spots, well distributed, and with good quality. The extraction process that associates successive washes with ultrasonication steps proved to be efficient for the detection of proteins. With this technique it was possible to obtain clear gels which were detected in about 600 spots distributed throughout the gel without the presence of drag.

Key words

Proteins Extraction Virus 2D PAGE 

Notes

Acknowledgments

This work was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq), process N° 304536/2015-6.

References

  1. 1.
    Cantú MD, Mariano AG, Palma MS et al (2008) Proteomic analysis reveals suppression of bark chitinases and proteinase inhibitors in citrus plants affected by the citrus sudden death disease. Phytopathology 98:1084–1092CrossRefGoogle Scholar
  2. 2.
    Chen SX, Harmon AC (2006) Advances in plant proteomic. Proteomics 6:5504–5516CrossRefGoogle Scholar
  3. 3.
    Newton RP, Brenton AG, Smith CJ et al (2004) Plant proteome analysis by mass spectrometry: principles, problems, pitfalls and recent developments. Phytochemistry 65:1449–1485CrossRefGoogle Scholar
  4. 4.
    Bar-Joseph M, Lee RF (1989) Citrus tristeza virus. AAB Descr Pl Viruses 353:7Google Scholar
  5. 5.
    Dória MS, de Sousa AO, Barbosa CJ et al (2015) Citrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety “Westin”. PLoS One 10:7CrossRefGoogle Scholar
  6. 6.
    Pirovani CP, Carvalho HAS, Machado RCR et al (2008) Protein extraction for proteome analysis from cacao leaves and meristems, organs infected by Moniliophthora perniciosa, the causal agent of the witches' broom disease. Electrophoresis 29:2391–2401CrossRefGoogle Scholar
  7. 7.
    Bertolde FZ, Almeida AA, Silva FA et al (2014) Efficient method of protein extraction from Theobroma cacao L. roots for two-dimensional gel electrophoresis and mass spectrometry analyses. Genet Mol Res 13:5036–5047CrossRefGoogle Scholar
  8. 8.
    Chalmers MJ, Gaskell SJ (2000) Advances in mass spectrometry for proteome analysis. Curr Opin Biotechnol 1:384–390CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Milena Santos Dória
    • 1
  • Carlos Priminho Pirovani
    • 1
  1. 1.Centro de Biotecnologia e GenéticaUniversidade Estadual de Santa CruzIlhéusBrazil

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