The Probiotic Mixture VSL#3 Reverses Olanzapine-Induced Metabolic Dysfunction in Mice

  • Navneet Dhaliwal
  • Jatinder Dhaliwal
  • Dhirendra Pratap Singh
  • Kanthi Kiran KondepudiEmail author
  • Mahendra Bishnoi
  • Kanwaljit Chopra
Part of the Methods in Molecular Biology book series (MIMB, volume 2011)


Atypical antipsychotic drugs (AAPDs) such as olanzapine (OLZ) are associated with serious metabolic adverse effects such as weight gain, visceral fat accretion, glucose intolerance, and lipid abnormalities. Compelling evidence suggests that the gut microbiota is known to regulate metabolic homeostasis and therefore microbiota-modulating strategies such as probiotics may serve as an excellent approach for treating metabolic adverse effects associated with atypical antipsychotic drugs (AAPDs). The therapeutic potential of VSL#3 (20 × 109 CFU/day), in reversing olanzapine-induced metabolic dysfunction, was assessed. VSL#3 administration led to attenuation of OLZ-induced body weight gain, uterine fat deposition, impaired glucose tolerance, and insulin resistance. Moreover olanzapine treatment also decreased inflammatory markers, abolished oxidative stress in the vWAT, and prevented shifts in gut microbiota abundance levels. These results indicate that VSL#3 via its ability to manipulate the gut microbiome confers beneficial metabolic effects and may, therefore, represent a novel therapeutic approach for reversing antipsychotic-induced metabolic dysfunction.

Key words

Adiponectin HOMA-IR Olanzapine Schizophrenia VSL#3 



The authors would like to acknowledge Prof Kanwaljit Chopra, Dr Mahendra Bishnoi, and Dr Kanti Kiran Kondepudi for their guidance and support.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Navneet Dhaliwal
    • 1
  • Jatinder Dhaliwal
    • 1
  • Dhirendra Pratap Singh
    • 1
    • 2
  • Kanthi Kiran Kondepudi
    • 2
    Email author
  • Mahendra Bishnoi
    • 2
  • Kanwaljit Chopra
    • 1
  1. 1.Pharmacology Division, University Institute of Pharmaceutical Sciences (UIPS)Punjab UniversityChandigarhIndia
  2. 2.National Agri-food Biotechnology Institute (NABI)MohaliIndia

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