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Methods in Electrode Implantation and Wiring for Long-Term Continuous EEG Monitoring in Rodent Models of Epilepsy and Behavioral Disturbances

  • Yasser Medlej
  • Houssein Salah
  • Lara Wadi
  • Zahraa Atoui
  • Yasser Fadlallah
  • Rita Asdikian
  • Rana Bou Khalil
  • Rabih Hashash
  • Makram ObeidEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2011)

Abstract

Rodent seizure models that pathologically and behaviorally recapitulate age-tailored epileptic disorders are used by us and others to advance our understanding of the chronobiology and mechanisms of epileptic seizure emergence and their comorbidities and to investigate potential novel treatment strategies. Obtaining prolonged continuous electroencephalogram (EEG) tracings over months is essential in this line of translational research, particularly to assess the relation between electrographic changes and the development of seizures and their various psychiatric and cognitive comorbidities in models where seizures gradually emerge over weeks following brain insults. Here we describe our approach to electrode implantation and wiring in order to successfully obtain high-quality continuous EEG tracings in rats for prolonged periods. A detailed stepwise methodological description is provided with a special focus on the details that help most in avoiding notorious pitfalls such as premature EEG cable disconnections and a poor signal to noise ratio.

Key words

Electroencephalogram Long-term continuous EEG Psychiatric comorbidities Epilepsy Rodents 

Notes

Acknowledgments

Yasser Fadlallah was involved in this research under the Medical Research Volunteer Program (MRVP) at the American University of Beirut.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yasser Medlej
    • 1
  • Houssein Salah
    • 1
  • Lara Wadi
    • 2
  • Zahraa Atoui
    • 2
  • Yasser Fadlallah
    • 3
  • Rita Asdikian
    • 1
  • Rana Bou Khalil
    • 4
  • Rabih Hashash
    • 4
  • Makram Obeid
    • 1
    • 5
    Email author
  1. 1.Department of Anatomy, Cell Biology and PhysiologyAmerican University of BeirutBeirutLebanon
  2. 2.Faculty of MedicineAmerican University of BeirutBeirutLebanon
  3. 3.Faculty of Arts and SciencesAmerican University of BeirutBeirutLebanon
  4. 4.Animal Care FacilityAmerican University of BeirutBeirutLebanon
  5. 5.Division of Child Neurology, Department of Pediatric and Adolescent MedicineAmerican University of Beirut Medical CenterBeirutLebanon

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