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Trypsiligase-Catalyzed Peptide and Protein Ligation

  • Sandra Liebscher
  • Frank BordusaEmail author
Protocol
  • 728 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2012)

Abstract

Site-specific incorporation of nonproteinogenic functionalities into protein targets is an important tool in both basic and applied research and represents a major challenge to protein chemists. Chemical labeling methods often target multiple positions within a protein and therefore suffer from a lack of specificity. Enzymatic protein modification is an attractive alternative due to the inherent regioselectivity and stereoselectivity of enzymes. In this chapter we describe the application of the highly specific trypsin variant trypsiligase for the site-specific modification of virtual any target protein. We present two general routes of modification resulting in either N- or C-terminal functionalized protein products. Reactions rapidly proceed under mild conditions and result in homogeneously modified proteins bearing the artificial functionality exclusively at the desired position. We detail protocols for the expression and purification of trypsiligase as well as the synthesis of peptide (ester) substrates. In addition, we provide instructions for the bioconjugation reactions and for the qualitative and quantitative analysis of reaction progress and efficiency.

Key words

Trypsin variant Peptide ligation Protein modification Substrate-assisted catalysis Substrate mimetic Transpeptidation Trypsiligase 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Biochemistry/Biotechnology, Charles-Tanford-Protein CenterMartin-Luther-University Halle-WittenbergHalleGermany

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