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In Vivo Measurement of H2S, Polysulfides, and “SSNO− Mix”-Mediated Vasoactive Responses and Evaluation of Ten Hemodynamic Parameters from Rat Arterial Pulse Waveform

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Vascular Effects of Hydrogen Sulfide

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2007))

Abstract

The chapter describes protocols and pitfalls in in vivo studies of drug effects on anesthetized rats. It focuses on the preparation of Na2S, Na2S4, and “SSNO− mix” solutions for rat intravenous administration, surgical preparation of jugular vein for drug administration, and preparation of carotid and tail arteries for recording of arterial pulse waveform (APW) at high resolution. It describes evaluation of ten hemodynamic parameters from APW and measurement of apparent pulse wave velocity.

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Acknowledgments

This work was supported by the Slovak Research and Development Agency under contract No. APVV-15-0371 and financial support by Slovak grants VEGA 2/0067/13, 2/0048/17, 2/0079/19, Ministry of Health of the Slovak Republic under the project registration number 2012/51-SAV-1 and APVV-15-0565.

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Authors and Affiliations

  1. Institute of Normal and Pathological Physiology, Centre of Experimental Medicine, Slovak Academy of Sciences, Bratislava, Slovakia

    Frantisek Kristek

  2. Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia

    Marian Grman & Karol Ondrias

Authors
  1. Frantisek Kristek
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  2. Marian Grman
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  3. Karol Ondrias
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Corresponding author

Correspondence to Frantisek Kristek .

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Editors and Affiliations

  1. Department of Pathophysiology, Medical University, Lublin, Poland

    Jerzy Bełtowski

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Kristek, F., Grman, M., Ondrias, K. (2019). In Vivo Measurement of H2S, Polysulfides, and “SSNO− Mix”-Mediated Vasoactive Responses and Evaluation of Ten Hemodynamic Parameters from Rat Arterial Pulse Waveform. In: Bełtowski, J. (eds) Vascular Effects of Hydrogen Sulfide. Methods in Molecular Biology, vol 2007. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9528-8_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9528-8_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9527-1

  • Online ISBN: 978-1-4939-9528-8

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