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Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue

  • Joris A. van Dongen
  • Martin C. Harmsen
  • Hieronymus P. Stevens
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1993)

Abstract

Adipose tissue-derived stromal cells (ASCs) are a promising candidates for cellular therapy in the field of regenerative medicine. ASCs are multipotent mesenchymal stem cell-like and reside in the stromal vascular fraction (SVF) of adipose tissue with the capacity to secrete a plethora of pro-regenerative growth factors. Future applications of ASCs may be restricted through (trans)national governmental policies that do not allow for use of nonhuman-derived (non-autologous) enzymes to isolate ASC. Besides, enzymatic isolation procedures are also time consuming. To overcome this issue, nonenzymatic isolation procedures to isolate ASCs or the SVF are being developed, such as the fractionation of adipose tissue procedure (FAT). This standardized procedure to isolate the stromal vascular fraction can be performed within 10–12 min. The short procedure time allows for intraoperative isolation of 1 mL of stromal vascular fraction derived from 10 mL of centrifuged adipose tissue. The stromal vascular fraction mostly contains blood vessels, extracellular matrix, and ASCs. However, based on the histological stainings an interdonor variation exists which might result in different therapeutic effects. The existing interdonor variations can be addressed by histological stainings and flow cytometry.

Key words

Stromal vascular fraction Adipose tissue Adipose tissue-derived stromal cells Fractionation Regenerative medicine Cell therapy 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Joris A. van Dongen
    • 1
    • 2
  • Martin C. Harmsen
    • 2
  • Hieronymus P. Stevens
    • 1
  1. 1.Plastic Surgery DepartmentVelthuis KliniekRotterdamThe Netherlands
  2. 2.Department of Pathology and Medical BiologyUniversity of Groningen and University Medical Centre of GroningenGroningenThe Netherlands

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