Human Reconstructed Skin in a Mouse Model

  • Jun Mi
  • Shuai Chen
  • Lin Xu
  • Jie Wen
  • Xin Xu
  • Xunwei Wu
Part of the Methods in Molecular Biology book series (MIMB, volume 1993)


Currently, no ideal in vivo skin model, to exactly mimic the native human skin, has been utilized for laboratory and clinical application. Here, we describe a method to in vivo reconstitute a human skin model, so-called hRSK, by using culture-expanded skin cells. We grafted a mixture of dissociated human epidermal and dermal cells onto an excision wound on the back of immunodeficient mouse to generate the hRSK, and the hRSK, containing epidermis, dermis, and subcutis and also appendages such as hair follicles, histologically mirrors in situ human skin.

Key words

Mouse model Skin epidermal cells Skin dermal cells Human reconstructed skin Cell grafting 



This work was supported by National Key Research and Development Program of China (2017YFA0104604), General Program of National Natural Science Foundation of China (81772093), and Shandong Taishan Scholar Award (tshw201502065).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jun Mi
    • 1
    • 2
  • Shuai Chen
    • 3
  • Lin Xu
    • 1
    • 4
  • Jie Wen
    • 1
  • Xin Xu
    • 1
  • Xunwei Wu
    • 1
    • 2
  1. 1.Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of StomatologyShandong UniversityJinanChina
  2. 2.Cutaneous Biology Research CenterMassachusetts General Hospital/Harvard Medical SchoolBostonUSA
  3. 3.Department of General Surgery and Neonatal SurgeryQilu Children’s Hospital of Shandong UniversityShandongChina
  4. 4.Department of StomatologyLiaocheng People’s HospitalShandongChina

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